全文获取类型
收费全文 | 3349篇 |
免费 | 360篇 |
国内免费 | 486篇 |
出版年
2023年 | 55篇 |
2022年 | 69篇 |
2021年 | 121篇 |
2020年 | 149篇 |
2019年 | 168篇 |
2018年 | 136篇 |
2017年 | 162篇 |
2016年 | 144篇 |
2015年 | 151篇 |
2014年 | 226篇 |
2013年 | 249篇 |
2012年 | 149篇 |
2011年 | 163篇 |
2010年 | 154篇 |
2009年 | 181篇 |
2008年 | 172篇 |
2007年 | 184篇 |
2006年 | 166篇 |
2005年 | 150篇 |
2004年 | 118篇 |
2003年 | 106篇 |
2002年 | 71篇 |
2001年 | 98篇 |
2000年 | 83篇 |
1999年 | 61篇 |
1998年 | 56篇 |
1997年 | 58篇 |
1996年 | 45篇 |
1995年 | 38篇 |
1994年 | 42篇 |
1993年 | 41篇 |
1992年 | 35篇 |
1991年 | 41篇 |
1990年 | 30篇 |
1989年 | 21篇 |
1988年 | 25篇 |
1987年 | 18篇 |
1986年 | 23篇 |
1985年 | 30篇 |
1984年 | 27篇 |
1983年 | 26篇 |
1982年 | 39篇 |
1981年 | 32篇 |
1980年 | 17篇 |
1979年 | 22篇 |
1978年 | 8篇 |
1977年 | 6篇 |
1976年 | 4篇 |
1974年 | 4篇 |
1972年 | 8篇 |
排序方式: 共有4195条查询结果,搜索用时 828 毫秒
71.
Longest root elongation diminished significantly in the three species tested from 6 mm d-1 to 3 mm d-1 in 3 weeks. During this period S.D. increased considerably (from 49% to 112%, A. castellana), and accounted on the average for 68% (A. capillaris) till 94% (A. castellana) of the mean. Maximum root growth stabilized at 6 mm d-1 and showed less variation in the measurements (S.D. 52% of the mean). Growth of the originally longest root approaches zero in all three species, in accordance with the natural cease of growth of roots in grasses fascicular root system. Measuring maximum root growth instead of longest root elongation is proposed for testing metal tolerance of grasses in sequential experiments. 相似文献
72.
C. S. Wang B. S. Yandell J. J. Rutledge 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,85(1):79-88
Summary At least two common practices exist when a negative variance component estimate is obtained, either setting it to zero or not reporting the estimate. The consequences of these practices are investigated in the context of the intraclass correlation estimation in terms of bias, variance and mean squared error (MSE). For the one-way analysis of variance random effects model and its extension to the common correlation model, we compare five estimators: analysis of variance (ANOVA), concentrated ANOVA, truncated ANOVA and two maximum likelihood-like (ML) estimators. For the balanced case, the exact bias and MSE are calculated via numerical integration of the exact sample distributions, while a Monte Carlo simulation study is conducted for the unbalanced case. The results indicate that the ANOVA estimator performs well except for designs with family size n = 2. The two ML estimators are generally poor, and the concentrated and truncated ANOVA estimators have some advantages over the ANOVA in terms of MSE. However, the large biases may make the concentrated and truncated ANOVA estimators objectionable when intraclass correlation () is small. Bias should be a concern when a pooled estimate is obtained from the literature since <0.05 in many genetic studies. 相似文献
73.
Direct screening for high-level expression of an introduced α-amylase gene in plants 总被引:2,自引:0,他引:2
Jan Pen Albert J. J. van Ooyen Peter J. M. van den Elzen Krijn Rietveld André Hoekema 《Plant molecular biology》1992,18(6):1133-1139
A method is described for obtaining transgenic plants with a high level of expression of the introduced gene. Tobacco protoplasts were transformed with an expression construct containing a translational fusion between mature -amylase from Bacillus licheniformis and the signal peptide of the tobacco PR-S protein. A total number of 5200 transformed protoplasts was cultured to microcalli and screened for -amylase expression by incubation on media containing starch followed by staining with iodine. The calli were divided into four classes, based on the resulting halo sizes on the plates. The halo sizes were found to correlated with the expression levels in transgenic plants regenerated from the calli. The expression levels varied between 0 and 0.5% of soluble leaf protein in the regenerated transgenic plants. Wider implications of this method are discussed. 相似文献
74.
Optimal production of glutathione by controlling the specific growth rate of yeast in fed-batch culture 总被引:8,自引:0,他引:8
The optimal of the specific growth rate was obtained with simple mathematical model in a yeast fed-batch cultures. The model was based on the mass balance around the fed-batch system and the relationship between the specific growth rate, mu, and the specific production rate of glutathione, rho(G). The optimal profile of mu was calculated as a bang-bang type, That is mu, should start from the maximum value, mu(max) and should be kept at mu(max); then mu should be switched to mu(c), which gives a maximum value of rho(G). It was proven from the maximum principle that switching was needed only once, with the switching time from mu(max) to mu(c) depending on the final required glutathione content. Finally, this ideal profile of mu for the maximum production of glutathione was realized by manipulating the substrates feed rate in the fed-batch culture. Using the extended Kalman filter and a programmed-controller/feedback-compensator (PF) system, mu could be controlled at the optimal profile obtained. As a result, the maximum production of glutathione was accomplished fairly successfully. However, further improvement in the controller performance for mu is desired. The control strategy employed here can be applied to other batch reaction processes. 相似文献
75.
76.
J. P. R. Ochieng'-Odero 《Entomologia Experimentalis et Applicata》1990,54(1):21-27
Adult reproductive performance is linked to the period of feeding done by the final instar larva after attainment of a larval critical weight (LCW). The highest weight attained by a final instar larva is referred to as the larval maximum weight (LMW) and is the onset of the pre-pupal period. The relationships between LCW, pupal weight (PW) and adult weight (AW) are described as functions of the LMW. In the leafroller Cnephasia jactatana (Walker) (Lepidoptera: Tortricidae) LCW was dependent on larval size and was approximately 75% of the mean LMW. LCW was about 29 mg and 36 mg for male and female larvae of 1.18 mm and 1.20 mm head-capsule width, respectively. Over three successive generations of laboratory rearing, PW was approximately 30% and 25% lower than the LMW for males and females, respectively. AW was consistently about 50% and 40% lower than the PW for males and females, respectively. The decrease in weight from LMW to PW was named as the constant DP and found to be 0.3 for males and 0.25 for females. The total decrease from LMW to AW was the constant DA and was 0.6 for males and 0.5 for females. The duration of the latent feeding period was positively correlated to PW and AW. LCW may be used to derive quality indices that describe and predict pupal and adult performance.
Résumé Les performances reproductives sont liées à la période d'alimentation du dernier stade larvaire après l'obtention d'un poids critique (LCW). Le poids maximal atteint au dernier stade larvaire est défini comme le poids larvaire maximal (LMW), il correspond au début de la période prénymphale. Les relations entre poids de la chrysalide (PW) et poids de l'audulte AW) sont présentées comme des fonctions de LMW). Chez Cnephasia jactatana Walker (Lep. Tortricidae), LCW dépend de la taille de la chenille et correspond à environ 75% de la valeur moyenne de LMW. LCW est respectivement de 29 mg et 36 mg pour les chenilles mâles et femelles dont les capsules céphaliques ont 1,18 et 1,20 mm. Pour 3 générations successives, PW est environ 30% et 25% inférieur à LMW des mâles et des femelles. La régression du poids de LMW à PW est désignée comme la constante DP et vaut 0,3 pour les mâles et 0,25 pour les femelles. La régression de LMW à AW est désignée comme la régression DM et vaut 0,6 pour les mâles et 0,5 pour les femelles. La durée de la période d'alimentation latente est liée positivement à PW et AW. LCW peut être utilisé comme indice dérivé décrivant et prédisant les potentialités nymphales et imaginales.相似文献
77.
Katsumi Ishida Ikuo Yasumasu 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1982,720(3):266-273
The level of adenosine 3′,5′-monophosphate (cyclic AMP) in the eggs of the sea urchin, Anthocidaris crassispina, was found to change periodically after fertilization. The minimum and maximum levels of cyclic AMP were 1.0·10?7 M and 1.5·10?6 M, respectively. The activity of adenylate cyclase in a 105 000 × g precipitate reached a plateau at 20 min after fertilization and stayed constant for at least 2 h. It was also found that 1.0 mM CaCl2 increased the activity of adenylate cyclase in the same precipitate from unfertilized eggs. In contrast, phosphodiesterase activity changed periodically and correlated with cyclic AMP levels in the eggs. Up to a concentration of 1.5·10?6 M cyclic AMP, phosphodiesterase activity was low, but it became activated when the level of cyclic AMP rose beyond this level. These results indicate that the change in the intracellular level of cyclic AMP is regulated mainly by the change in phosphodiesterase activity. 相似文献
78.
79.
Addition of ATP to chloroplasts causes a reversible 25–30% decrease in chlorophyll fluorescence. This quenching is light-dependent, uncoupler insensitive but inhibited by DCMU and electron acceptors and has a half-time of 3 minutes. Electron donors to Photosystem I can not overcome the inhibitory effect of DCMU, suggesting that light activation depends on the reduced state of plastoquinone. Fluorescence emission spectra recorded at ?196°C indicate that ATP treatment increases the amount of excitation energy transferred to Photosystem I. Examination of fluorescence induction curves indicate that ATP treatment decreases both the initial (Fo) and variable (Fv) fluorescence such that the ratio of Fv to the maximum (Fm) yield is unchanged. The initial sigmoidal phase of induction is slowed down by ATP treatment and is quenched 3-fold more than the exponential slow phase, the rate of which is unchanged. A plot of Fv against area above the induction curve was identical plus or minus ATP. Thus ATP treatment can alter quantal distribution between Photosystems II and I without altering Photosystem II-Photosystem II interaction. The effect of ATP strongly resembles in its properties the phosphorylation of the light-harvesting complex by a light activated, ATP-dependent protein kinase found in chloroplast membranes and could be the basis of physiological mechanisms which contribute to slow fluorescence quenching in vivo and regulate excitation energy distribution between Photosystem I and II. It is suggested that the sensor for this regulation is the redox state of plastoquinone. 相似文献
80.
Summary Using bioluminescence of luciferin-luciferase, we showed that ATP leaked out rhythmically from a strand segment ofPhysarum plasmodium made permeable with caffeine-arsenate. With simultaneous measurement of isometric tension rhythm of the strand, it was revealed that the period and phase of oscillation in ATP leakage correspond well with those of tension production. Further, microinjection of luciferin-luciferase into the plasmodial strand indicated that the intracellular luminescence of luciferin-luciferase also oscillates with the same period and in the same phase as the tension rhythm.The free ATP concentration in a homogenate ofPhysarum plasmodium was of the order of 10 M, but if the homogenate was heated in boiling water, the intensity of luminescence suddenly increased 10–100 fold. ATP available for mechanical workin vivo is thus supposed to be at a much lower level than the total average, which was found in the range of 0.2–0.7 mM. 相似文献